MAD SCIENCE – LUCIFERASE, CRISPR, mRNA….

THE MARSHALL REPORT

WHAT COULD POSSIBLY GO WRONG?

Abstract

The luciferase gene from the firefly,Photinus pyralis, was used as a reporter of gene expression by light production in transfected plant cells and transgenic plants. A complementary DNA clone of the firefly luciferase gene under the control of a plant virus promoter (cauliflower mosaic virus 35SRNA promoter) was introduced into plant protoplast cells (Daucus carota) by electroporation and into plants (Nicotiana tabacum) by use of theAgrobacterium tumefacienstumor-inducing plasmid. Extracts from electroporated cells (24 hours after the introduction of DNA) and from transgenic plants produce light when mixed with the substrates luciferin and adenosine triphosphate.

MIT engineers just unveiled living, glowing plants
MIT engineers just unveiled living, glowing plantsMIT engineers just unveiled living, glowing plants (inhabitat.com)

Light produced by the action of luciferase was also detected in undisrupted leaves or cells in culture from transgenic plants incubated in luciferin and…

View original post 1,203 more words

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google photo

You are commenting using your Google account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s